Phosphopeptide Characterization by Mass Spectrometry using Reversed-Phase Supports for Solid-Phase β-Elimination/Michael Addition

We have adapted the Ba(2+) ion-catalyzed concurrent Michael addition reaction to solid-phase derivatization on ZipTip(C18) pipette tips using 2-aminoethanethiol as a nucleophile. This approach provides several advantages over the classical in-solution-based techniques, including ease of operation, c...

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Autores principales: Nika, Heinz, Lee, JaeHoon, Willis, Ian M., Angeletti, Ruth Hogue, Hawke, David H.
Formato: Artigo
Lenguaje:Inglês
Publicado: Association of Biomolecular Resource Facilities 2012
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3324170/
https://ncbi.nlm.nih.gov/pubmed/22951960
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7171/jbt.2012-2302-002
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