Cleavage of chromatin with methidiumpropyl-EDTA . iron(II).
Methidiumpropyl-EDTA . iron(II) [MPE . Fe (II)] cleaves double-helical DNA with considerably lower sequence specificity than micrococcal nuclease. Moreover, digestions with MPE . Fe(II) can be performed in the presence of certain metal chelators, which will minimize the action of many endogenous nuc...
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| Veröffentlicht in: | Proc Natl Acad Sci U S A |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
1983
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC394010/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6407008/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.11.3213 |
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