Cleavage of chromatin with methidiumpropyl-EDTA . iron(II).

Methidiumpropyl-EDTA . iron(II) [MPE . Fe (II)] cleaves double-helical DNA with considerably lower sequence specificity than micrococcal nuclease. Moreover, digestions with MPE . Fe(II) can be performed in the presence of certain metal chelators, which will minimize the action of many endogenous nuc...

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Autors principals: Cartwright, I L, Hertzberg, R P, Dervan, P B, Elgin, S C
Format: Artigo
Idioma:Inglês
Publicat: 1983
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC394010/
https://ncbi.nlm.nih.gov/pubmed/6407008
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