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Cleavage of chromatin with methidiumpropyl-EDTA . iron(II).

Methidiumpropyl-EDTA . iron(II) [MPE . Fe (II)] cleaves double-helical DNA with considerably lower sequence specificity than micrococcal nuclease. Moreover, digestions with MPE . Fe(II) can be performed in the presence of certain metal chelators, which will minimize the action of many endogenous nuc...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Cartwright, I L, Hertzberg, R P, Dervan, P B, Elgin, S C
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1983
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC394010/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6407008/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.11.3213
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