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High mutation frequency in DNA transfected into mammalian cells.

The lacI gene of Escherichia coli was used to score mutation in mammalian cells of simian virus 40-based recombinant DNA vectors that provide for replication and selection in both bacterial and mammalian cells. Plasmid DNA was introduced into COS7 simian cells by DEAE-dextran transfection, allowed t...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Calos, M P, Lebkowski, J S, Botchan, M R
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1983
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393964/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6574469/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.10.3015
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