High mutation frequency in DNA transfected into mammalian cells.
The lacI gene of Escherichia coli was used to score mutation in mammalian cells of simian virus 40-based recombinant DNA vectors that provide for replication and selection in both bacterial and mammalian cells. Plasmid DNA was introduced into COS7 simian cells by DEAE-dextran transfection, allowed t...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1983
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393964/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6574469/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.10.3015 |
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