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High-throughput de novo screening of receptor agonists with an automated single-cell analysis and isolation system
Reconstitution of signaling pathways involving single mammalian transmembrane receptors has not been accomplished in yeast cells. In this study, intact EGF receptor (EGFR) and a cell wall-anchored form of EGF were co-expressed on the yeast cell surface, which led to autophosphorylation of the EGFR i...
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| Main Authors: | , , , , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Nature Publishing Group
2014
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3937795/ https://ncbi.nlm.nih.gov/pubmed/24577528 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep04242 |
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