Assessment of Myeloperoxidase Activity by the Conversion of Hydroethidine to 2-Chloroethidium

Oxidants derived from myeloperoxidase (MPO) contribute to inflammatory diseases. In vivo MPO activity is commonly assessed by the accumulation of 3-chlorotyrosine (3-Cl-Tyr), although 3-Cl-Tyr is formed at low yield and is subject to metabolism. Here we show that MPO activity can be assessed using h...

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Main Authors: Maghzal, Ghassan J., Cergol, Katie M., Shengule, Sudhir R., Suarna, Cacang, Newington, Darren, Kettle, Anthony J., Payne, Richard J., Stocker, Roland
格式: Artigo
語言:Inglês
出版: American Society for Biochemistry and Molecular Biology 2014
主題:
Enzymology
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3937635/
https://ncbi.nlm.nih.gov/pubmed/24436331
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M113.539486
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