Validation and implementation of a method for microarray gene expression profiling of minor B-cell subpopulations in man

BACKGROUND: This report describes a method for the generation of global gene expression profiles from low frequent B-cell subsets by using fluorescence-activated cell sorting and RNA amplification. However, some of the differentiating compartments involve a low number of cells and therefore it is im...

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Hauptverfasser: Bergkvist, Kim Steve, Nyegaard, Mette, Bøgsted, Martin, Schmitz, Alexander, Bødker, Julie Støve, Rasmussen, Simon Mylius, Perez-Andres, Martin, Falgreen, Steffen, Bilgrau, Anders Ellern, Kjeldsen, Malene Krag, Gaihede, Michael, Nørgaard, Martin Agge, Bæch, John, Grønholdt, Marie-Louise, Jensen, Frank Svendsen, Johansen, Preben, Dybkær, Karen, Johnsen, Hans Erik
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2014
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3937209/
https://ncbi.nlm.nih.gov/pubmed/24483235
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2172-15-3
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