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Nucleotide selection by the Y-family DNA polymerase Dpo4 involves template translocation and misalignment

Y-family DNA polymerases play a crucial role in translesion DNA synthesis. Here, we have characterized the binding kinetics and conformational dynamics of the Y-family polymerase Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) using single-molecule fluorescence. We find that in the absence of dN...

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Autors principals: Brenlla, Alfonso, Markiewicz, Radoslaw P., Rueda, David, Romano, Louis J.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3936744/
https://ncbi.nlm.nih.gov/pubmed/24270793
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt1149
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