Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination

Synthetic biology requires effective methods to assemble DNA parts into devices and to modify these devices once made. Here we demonstrate a convenient rapid procedure for DNA fragment assembly using site-specific recombination by ϕC31 integrase. Using six orthogonal attP/attB recombination site pai...

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Hlavní autoři: Colloms, Sean D., Merrick, Christine A., Olorunniji, Femi J., Stark, W. Marshall, Smith, Margaret C. M., Osbourn, Anne, Keasling, Jay D., Rosser, Susan J.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2014
Témata:
Methods Online
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3936721/
https://ncbi.nlm.nih.gov/pubmed/24225316
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt1101
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