Crystallization and preliminary X-ray diffraction analysis of the homing endonuclease I-CvuI from Chlorella vulgaris in complex with its target DNA

Homing endonucleases are highly specific DNA-cleaving enzymes that recognize long stretches of DNA. The engineering of these enzymes provides novel instruments for genome modification in a wide range of fields, including gene targeting, by inducing specific double-strand breaks. I-CvuI is a homing e...

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Bibliographic Details
Main Authors: Redondo, Pilar, Merino, Nekane, Villate, Maider, Blanco, Francisco J., Montoya, Guillermo, Molina, Rafael
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2014
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3936450/
https://ncbi.nlm.nih.gov/pubmed/24637769
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X1400065X
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