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Promoter-proximal pausing by RNA polymerase II in vitro: transcripts shorter than 20 nucleotides are not capped.

We have synthesized RNA from cloned adenovirus 2 late promoter DNA in an in vitro transcription extract under UTP-limiting conditions. Under these circumstances, almost all of the alpha-amanitin-sensitive RNA produced is shorter than 20 nucleotides; most of these short transcripts are present in fou...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Coppola, J A, Field, A S, Luse, D S
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 1983
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393573/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6572384/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.5.1251
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