Promoter-proximal pausing by RNA polymerase II in vitro: transcripts shorter than 20 nucleotides are not capped.
We have synthesized RNA from cloned adenovirus 2 late promoter DNA in an in vitro transcription extract under UTP-limiting conditions. Under these circumstances, almost all of the alpha-amanitin-sensitive RNA produced is shorter than 20 nucleotides; most of these short transcripts are present in fou...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1983
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393573/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6572384/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.5.1251 |
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