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Isolation of a cDNA clone for human X-linked 3-phosphoglycerate kinase by use of a mixture of synthetic oligodeoxyribonucleotides as a detection probe.

We have obtained a cDNA clone encoding most of human X-linked 3-phosphoglycerate kinase (PGK; ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3). Total mRNA was prepared from human adenocarcinoma-derived cell line LS174T and used for cDNA preparation. Double-stranded cDNA was inserted, afte...

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Publicat a:Proc Natl Acad Sci U S A
Autors principals: Singer-Sam, J, Simmer, R L, Keith, D H, Shively, L, Teplitz, M, Itakura, K, Gartler, S M, Riggs, A D
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 1983
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393468/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6572369/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.3.802
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