Isolation of a cDNA clone for human X-linked 3-phosphoglycerate kinase by use of a mixture of synthetic oligodeoxyribonucleotides as a detection probe.

We have obtained a cDNA clone encoding most of human X-linked 3-phosphoglycerate kinase (PGK; ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3). Total mRNA was prepared from human adenocarcinoma-derived cell line LS174T and used for cDNA preparation. Double-stranded cDNA was inserted, afte...

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Detalhes bibliográficos
Main Authors: Singer-Sam, J, Simmer, R L, Keith, D H, Shively, L, Teplitz, M, Itakura, K, Gartler, S M, Riggs, A D
Formato: Artigo
Idioma:Inglês
Publicado em: 1983
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC393468/
https://ncbi.nlm.nih.gov/pubmed/6572369
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