Isolation of a cDNA clone for the human HLA-DR antigen alpha chain by using a synthetic oligonucleotide as a hybridization probe.

We have used a synthetic 20-nucleotide hybridization probe to isolate a cDNA clone encoding the alpha chain of the HLA-DR antigen from a cDNA library constructed from membrane-bound poly(A)+ mRNA. A set of synthetic 11-nucleotide fragments, potentially complementary to the codons for amino acids 11-...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Stetler, D, Das, H, Nunberg, J H, Saiki, R, Sheng-Dong, R, Mullis, K B, Weissman, S M, Erlich, H A
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1982
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347032/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6136966/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.19.5966
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