Isolation of a cDNA clone for the human HLA-DR antigen alpha chain by using a synthetic oligonucleotide as a hybridization probe.
We have used a synthetic 20-nucleotide hybridization probe to isolate a cDNA clone encoding the alpha chain of the HLA-DR antigen from a cDNA library constructed from membrane-bound poly(A)+ mRNA. A set of synthetic 11-nucleotide fragments, potentially complementary to the codons for amino acids 11-...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1982
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347032/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6136966/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.19.5966 |
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