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Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.

The deoxyribooligonucleotide, d(pT-T-A-G-C-A-G-A-A-C-C-G-G), constituting a segment of yeast iso-1-cytochrome c gene, has been synthesized by a combination of chemical and primarily enzymatic methods. The starting primer, d(pT-T-A-G1, was chemically synthesized by the phosphodiester method and was e...

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Библиографические подробности
Главные авторы: Gillam, S, Rottman, F, Jahnke, P, Smith, M
Формат: Artigo
Язык:Inglês
Опубликовано: 1977
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC393204/
https://ncbi.nlm.nih.gov/pubmed/189317
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