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Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.

The deoxyribooligonucleotide, d(pT-T-A-G-C-A-G-A-A-C-C-G-G), constituting a segment of yeast iso-1-cytochrome c gene, has been synthesized by a combination of chemical and primarily enzymatic methods. The starting primer, d(pT-T-A-G1, was chemically synthesized by the phosphodiester method and was e...

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Vydáno v:Proc Natl Acad Sci U S A
Hlavní autoři: Gillam, S, Rottman, F, Jahnke, P, Smith, M
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 1977
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393204/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/189317/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.1.96
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