Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Properties of the enzyme*
A modified purification is described for an enzyme, from Escherichia coli B, which polymerizes deoxyribonucleoside-5′ diphosphates. Under appropriate conditions, the enzyme will add a single deoxyribonucleotide residue to a deoxyribo-oligonucleotide primer. At all stages, the enzyme activity copurif...
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| Pubblicato in: | Nucleic Acids Res |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1974
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC343444/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4615301/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/1.12.1631 |
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