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Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Properties of the enzyme*
A modified purification is described for an enzyme, from Escherichia coli B, which polymerizes deoxyribonucleoside-5′ diphosphates. Under appropriate conditions, the enzyme will add a single deoxyribonucleotide residue to a deoxyribo-oligonucleotide primer. At all stages, the enzyme activity copurif...
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| Главные авторы: | , |
|---|---|
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
1974
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC343444/ https://ncbi.nlm.nih.gov/pubmed/4615301 |
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