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Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Properties of the enzyme*

A modified purification is described for an enzyme, from Escherichia coli B, which polymerizes deoxyribonucleoside-5′ diphosphates. Under appropriate conditions, the enzyme will add a single deoxyribonucleotide residue to a deoxyribo-oligonucleotide primer. At all stages, the enzyme activity copurif...

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Autors principals: Gillam, Shirley, Smith, Michael
Format: Artigo
Idioma:Inglês
Publicat: 1974
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC343444/
https://ncbi.nlm.nih.gov/pubmed/4615301
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