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A rationale for stabilization of oxygen-labile enzymes: application to a clostridial hydrogenase.

A general procedure for stabilization of O2-labile enzymes exploiting "salting out" of oxygen from the microenvironment in the molecular layers immediately adjacent to charged surfaces of polyionic solid adsorbents has been developed. Empirical verification of this rationale is provided. The half-li...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Klibanov, A M, Kaplan, N O, Kamen, M D
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1978
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392841/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/278979/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.8.3640
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