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Role of Active Site Residues in Promoting Cobalt-Carbon Bond Homolysis in Adenosylcobalamin-Dependent Mutases Revealed Through Experiment and Computation

Adenosylcobalamin serves as a source of reactive free radicals that are generated by homolytic scission of the coenzyme’s cobalt-carbon bond. AdoCbl-dependent enzymes accelerate AdoCbl homolysis by ~10(12)-fold, but the mechanism by which this is accomplished remains unclear. We have combined experi...

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Detalhes bibliográficos
Main Authors: Román-Meléndez, Gabriel D., von Glehn, Patrick, Harvey, Jeremy N., Mulholland, Adrian J., Marsh, E. Neil G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3928028/
https://ncbi.nlm.nih.gov/pubmed/24341954
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi4012644
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