Cold pepsin digestion: a novel method to produce the Fv fragment from human immunoglobulin M.
A strategy for the proteolytic fragmentation of human IgM has been developed. This method is called "cold pepsin digestion" because of its unique feature of achieving restricted peptic cleavages at 4 degrees and pH 4.0. Cold pepsin digestion has been applied successfully to produce an Fv fragment fr...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1978
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392620/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/351613/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.6.2649 |
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