PCR with 5-methyl-dCTP replacing dCTP.
When dCTP is replaced by methyl5-dCTP in the polymerase chain reaction some templates cannot be efficiently amplified by Taq polymerase or Vent polymerase using standard cycling parameters. However, this phenomenon can be overcome by increasing the temperature of the denaturation steps to 100 degree...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1991
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC333784/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1850509/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/19.5.1081 |
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