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PCR with 5-methyl-dCTP replacing dCTP.

When dCTP is replaced by methyl5-dCTP in the polymerase chain reaction some templates cannot be efficiently amplified by Taq polymerase or Vent polymerase using standard cycling parameters. However, this phenomenon can be overcome by increasing the temperature of the denaturation steps to 100 degree...

詳細記述

保存先:
書誌詳細
主要な著者: Wong, K K, McClelland, M
フォーマット: Artigo
言語:Inglês
出版事項: 1991
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC333784/
https://ncbi.nlm.nih.gov/pubmed/1850509
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