Construction of a novel plasmid-phage hybrid: use of the hybrid to demonstrate ColE1 DNA replication in vivo in the absence of a ColE1-specified protein.
A hybrid bacteriophage, P420, was constructed in vitro; it contains part of bacteriophage P4 and a 3.6-kilobase derivative of plasmid ColE1. In Escherichia coli, the plasmid-phage hybrid can exist as a stable plasmid or can be packaged into infective bacteriophage particles. Replication of P420, dir...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1978
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392519/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/276860/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.5.2200 |
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