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Construction of a novel plasmid-phage hybrid: use of the hybrid to demonstrate ColE1 DNA replication in vivo in the absence of a ColE1-specified protein.

A hybrid bacteriophage, P420, was constructed in vitro; it contains part of bacteriophage P4 and a 3.6-kilobase derivative of plasmid ColE1. In Escherichia coli, the plasmid-phage hybrid can exist as a stable plasmid or can be packaged into infective bacteriophage particles. Replication of P420, dir...

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Vydáno v:Proc Natl Acad Sci U S A
Hlavní autoři: Kahn, M, Helinski, D R
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 1978
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On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392519/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/276860/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.5.2200
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