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Construction of a novel plasmid-phage hybrid: use of the hybrid to demonstrate ColE1 DNA replication in vivo in the absence of a ColE1-specified protein.

A hybrid bacteriophage, P420, was constructed in vitro; it contains part of bacteriophage P4 and a 3.6-kilobase derivative of plasmid ColE1. In Escherichia coli, the plasmid-phage hybrid can exist as a stable plasmid or can be packaged into infective bacteriophage particles. Replication of P420, dir...

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Dettagli Bibliografici
Autori principali: Kahn, M, Helinski, D R
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1978
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC392519/
https://ncbi.nlm.nih.gov/pubmed/276860
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