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Growth of embryonal carcinoma cells in serum-free medium.
Two mouse embryonal carcinoma cell lines, PCC.4 aza-1 and F9, have been grown in serum-free F-12 medium supplemented with Pedersen fetuin, insulin, transferrin, and 2-mercaptoethanol. This medium supports long-term growth of both cell lines. When these cells are transferred from medium containing se...
Enregistré dans:
| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1978
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392437/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/77018/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.4.1844 |
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