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Growth of embryonal carcinoma cells in serum-free medium.

Two mouse embryonal carcinoma cell lines, PCC.4 aza-1 and F9, have been grown in serum-free F-12 medium supplemented with Pedersen fetuin, insulin, transferrin, and 2-mercaptoethanol. This medium supports long-term growth of both cell lines. When these cells are transferred from medium containing se...

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Foilsithe in:Proc Natl Acad Sci U S A
Main Authors: Rizzino, A, Sato, G
Formáid: Artigo
Teanga:Inglês
Foilsithe: National Academy of Sciences 1978
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392437/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/77018/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.4.1844
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