5′-Deoxy-5′-Hydrazinylguanosine as an Initiator of T7 RNA Polymerase-Catalyzed Transcriptions for the Preparation of Labeling-Ready RNAS

5′-deoxy-5′-hydrazinylguanosine was incorporated into the 5′-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5′-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay.

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Detalhes bibliográficos
Main Authors: Skipsey, Mark, Hack, Gordon, Hooper, Thomas A., Shankey, Mark C., Conway, Louis P., Schröder, Martin, Hodgson, David R. W.
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2013
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3924349/
https://ncbi.nlm.nih.gov/pubmed/24328564
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15257770.2013.851393
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