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Avidin-biotin affinity chromatography: application to the isolation of human placental insulin receptor.

The ligand N alpha, B1-(6-biotinylamido)hexanoyl-insulin was attached noncovalently to Sepharose 4B immobilized succinoylavidin to form an insulin-affinity resin. This resin was used to isolate highly purified insulin receptor from human placental tissue by a four step process involving (i) preparat...

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Vydáno v:Proc Natl Acad Sci U S A
Hlavní autoři: Finn, F M, Titus, G, Horstman, D, Hofmann, K
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 1984
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392139/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6390435/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.23.7328
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