Avidin-biotin affinity chromatography: application to the isolation of human placental insulin receptor.
The ligand N alpha, B1-(6-biotinylamido)hexanoyl-insulin was attached noncovalently to Sepharose 4B immobilized succinoylavidin to form an insulin-affinity resin. This resin was used to isolate highly purified insulin receptor from human placental tissue by a four step process involving (i) preparat...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1984
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392139/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6390435/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.23.7328 |
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