Conformational Cycle of the Vitamin B(12) ABC Importer in Liposomes Detected by Double Electron-Electron Resonance (DEER)

Double electron-electron resonance is used here to investigate intermediates of the transport cycle of the Escherichia coli vitamin B(12) ATP-binding cassette importer BtuCD-F. Previously, we showed the ATP-induced opening of the cytoplasmic gate I in TM5 helices, later confirmed by the AMP-PNP-boun...

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Detalhes bibliográficos
Main Authors: Joseph, Benesh, Korkhov, Vladimir M., Yulikov, Maxim, Jeschke, Gunnar, Bordignon, Enrica
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2014
Assuntos:
Protein Structure and Folding
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3916522/
https://ncbi.nlm.nih.gov/pubmed/24362024
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M113.512178
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