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A Vector Library for Silencing Central Carbon Metabolism Genes with Antisense RNAs in Escherichia coli
We describe here the construction of a series of 71 vectors to silence central carbon metabolism genes in Escherichia coli. The vectors inducibly express antisense RNAs called paired-terminus antisense RNAs, which have a higher silencing efficacy than ordinary antisense RNAs. By measuring mRNA amoun...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3911099/ https://ncbi.nlm.nih.gov/pubmed/24212579 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02376-13 |
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