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On the nature of the 5-lipoxygenase reaction in human leukocytes: enzyme purification and requirement for multiple stimulatory factors.
Arachidonate 5-lipoxygenase was purified 400-fold from homogenates of human peripheral blood leukocytes by a combination of ammonium sulfate fractionation, gel filtration chromatography, and HPLC on anion exchange and hydroxylapatite columns. NaDodSO4/polyacrylamide gel electrophoresis of the purifi...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1985
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390695/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3929248/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.18.6040 |
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