Mutagenesis of some conserved residues in human 5-lipoxygenase: effects on enzyme activity.
Recombinant human 5-lipoxygenase (arachidonate:oxygen 5-oxidoreductase, EC 1.13.11.34) was expressed in Escherichia coli. In incubations of E. coli supernatants with arachidonic acid, 5-hydroxy-7,9,11,14-eicosatetraenoic acid and leukotriene A4 were formed, while incubation with 8,11,14-eicosatrieno...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1992
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48263/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1731317/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.2.485 |
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