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Targeting endogenous nuclear antigens by electrotransfer of monoclonal antibodies in living cells

Antibodies are valuable tools for functional studies in vitro, but their use in living cells remains challenging because they do not naturally cross the cell membrane. Here, we present a simple and highly efficient method for the intracytoplasmic delivery of any antibody into cultured cells. By foll...

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Main Authors: Freund, Guillaume, Sibler, Annie-Paule, Desplancq, Dominique, Oulad-Abdelghani, Mustapha, Vigneron, Marc, Gannon, Julian, Van Regenmortel, Marc H., Weiss, Etienne
Formato: Artigo
Idioma:Inglês
Publicado: Landes Bioscience 2013
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3906305/
https://ncbi.nlm.nih.gov/pubmed/23765067
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/mabs.25084
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