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Electroporation of Labeled Antibodies to Visualize Endogenous Proteins and Posttranslational Modifications in Living Metazoan Cell Types

The spatiotemporal localization of different intracellular factors in real-time and their detection in live cells are important parameters to understand dynamic protein-based processes. Therefore, there is a demand to perform live-cell imaging and to measure endogenous protein dynamics in single cel...

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Bibliographische Detailangaben
Veröffentlicht in:Bio Protoc
Hauptverfasser: Conic, Sascha, Desplancq, Dominique, Tora, László, Weiss, Etienne
Format: Artigo
Sprache:Inglês
Veröffentlicht: Bio-Protocol 2018
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6245562/
https://ncbi.nlm.nih.gov/pubmed/30467550
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3069
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