Artificial genetic selection for an efficient translation initiation site for expression of human RACK1 gene in Escherichia coli

In bacterial expression systems, translation initiation is usually the rate limiting and the least predictable stage of protein synthesis. Efficiency of a translation initiation site can vary dramatically depending on the sequence context. This is why many standard expression vectors provide very po...

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Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Zhelyabovskaya, Olga B., Berlin, Yuri A., Birikh, Klara R.
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 2004
Assuntos:	NAR Methods Online
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390350/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15034151/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh050
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