Artificial genetic selection for an efficient translation initiation site for expression of human RACK1 gene in Escherichia coli

In bacterial expression systems, translation initiation is usually the rate limiting and the least predictable stage of protein synthesis. Efficiency of a translation initiation site can vary dramatically depending on the sequence context. This is why many standard expression vectors provide very po...

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Библиографические подробности

Опубликовано в::	Nucleic Acids Res
Главные авторы:	Zhelyabovskaya, Olga B., Berlin, Yuri A., Birikh, Klara R.
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Oxford University Press 2004
Предметы:	NAR Methods Online
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390350/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15034151/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh050
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