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Artificial genetic selection for an efficient translation initiation site for expression of human RACK1 gene in Escherichia coli
In bacterial expression systems, translation initiation is usually the rate limiting and the least predictable stage of protein synthesis. Efficiency of a translation initiation site can vary dramatically depending on the sequence context. This is why many standard expression vectors provide very po...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
2004
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC390350/ https://ncbi.nlm.nih.gov/pubmed/15034151 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh050 |
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