Targeted DNA methylation by homology-directed repair in mammalian cells. Transcription reshapes methylation on the repaired gene

We report that homology-directed repair of a DNA double-strand break within a single copy Green Fluorescent Protein (GFP) gene in HeLa cells alters the methylation pattern at the site of recombination. DNA methyl transferase (DNMT)1, DNMT3a and two proteins that regulate methylation, Np95 and GADD45...

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Hlavní autoři: Morano, Annalisa, Angrisano, Tiziana, Russo, Giusi, Landi, Rosaria, Pezone, Antonio, Bartollino, Silvia, Zuchegna, Candida, Babbio, Federica, Bonapace, Ian Marc, Allen, Brittany, Muller, Mark T., Chiariotti, Lorenzo, Gottesman, Max E., Porcellini, Antonio, Avvedimento, Enrico V.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2014
Témata:
Gene Regulation, Chromatin and Epigenetics
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3902918/
https://ncbi.nlm.nih.gov/pubmed/24137009
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt920
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