Definition of oriR, the minimum DNA segment essential for initiation of R1 plasmid replication in vitro.
The 3.6-kilobase Bgl II-EcoRI fragment from R1 plasmid containing copA, repA, and the replication origin (ori) was inserted into the ColE1-type plasmid pUC8. The resulting hybrid plasmid replicates in extracts prepared from both polA- and polA+ cells, whereas pUC8 replicates only in a polA+ extract....
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| Vydáno v: | Proc Natl Acad Sci U S A |
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| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1983
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390076/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6316326/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.22.6814 |
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