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Definition of oriR, the minimum DNA segment essential for initiation of R1 plasmid replication in vitro.

The 3.6-kilobase Bgl II-EcoRI fragment from R1 plasmid containing copA, repA, and the replication origin (ori) was inserted into the ColE1-type plasmid pUC8. The resulting hybrid plasmid replicates in extracts prepared from both polA- and polA+ cells, whereas pUC8 replicates only in a polA+ extract....

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Bibliografske podrobnosti
izdano v:Proc Natl Acad Sci U S A
Main Authors: Masai, H, Kaziro, Y, Arai, K
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 1983
Teme:
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390076/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6316326/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.22.6814
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