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Stopped-flow cryoenzymology: detection of catalytic intermediates not observable at ambient temperatures.

Stopped-flow cryoenzymology has been used to study the reaction of porcine kidney cytosol leucine aminopeptidase [alpha-aminoacyl-peptide hydrolase(cytosol), EC 3.4.11.1] with L-leucylglycyldansyl hydrazide in 50% (vol/vol) methanol buffer over the -40 to 23 degrees C temperature range. Resonance en...

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Detalles Bibliográficos
Autores principales: Van Wart, H E, Lin, S H
Formato: Artigo
Lenguaje:Inglês
Publicado: 1983
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC389980/
https://ncbi.nlm.nih.gov/pubmed/6584868
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