Cryoenzymology of trypsin. 13C-n.m.r. detection of an acyl-trypsin intermediate in the trypsin-catalysed hydrolysis of a highly specific substrate at subzero temperature.

The kinetics of the trypsin-catalysed hydrolysis of the highly specific substrate N alpha-benzyloxycarbonyl-L-lysine p-nitrophenyl ester were studied under cryoenzymological conditions by 13C-n.m.r. spectroscopy at pH approx. 3.0. The kinetics of this reaction are shown to be in agreement with simil...

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Bibliografske podrobnosti
Main Authors: Mackenzie, N E, Malthouse, J P, Scott, A I
Format: Artigo
Jezik:Inglês
Izdano: 1984
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1153500/
https://ncbi.nlm.nih.gov/pubmed/6430268
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