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Stopped-flow cryoenzymology: detection of catalytic intermediates not observable at ambient temperatures.
Stopped-flow cryoenzymology has been used to study the reaction of porcine kidney cytosol leucine aminopeptidase [alpha-aminoacyl-peptide hydrolase(cytosol), EC 3.4.11.1] with L-leucylglycyldansyl hydrazide in 50% (vol/vol) methanol buffer over the -40 to 23 degrees C temperature range. Resonance en...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1983
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC389980/ https://ncbi.nlm.nih.gov/pubmed/6584868 |
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