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In-depth qualitative and quantitative analysis of composite glycosylation profiles and other micro-heterogeneity on intact monoclonal antibodies by high-resolution native mass spectrometry using a modified Orbitrap

Here, we describe a fast, easy-to-use, and sensitive method to profile in-depth structural micro-heterogeneity, including intricate N-glycosylation profiles, of monoclonal antibodies at the native intact protein level by means of mass spectrometry using a recently introduced modified Orbitrap Exacti...

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Detalhes bibliográficos
Main Authors: Rosati, Sara, van den Bremer, Ewald TJ, Schuurman, Janine, Parren, Paul WHI, Kamerling, Johannis P, Heck, Albert JR
Formato: Artigo
Idioma:Inglês
Publicado em: Landes Bioscience 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3896605/
https://ncbi.nlm.nih.gov/pubmed/23995615
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/mabs.26282
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